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Figure 1. Example of Detection of pAkt and total Akt in Calyculin A-treated Jurkat lysate using Odyssey Blocking Buffer (TBS) as blocking agent. pAkt and total Akt were detected in calyculin A-stimulated (+) and non-stimulated (-) Jurkat lysate at 10 µp;g; 5 µp;g; and 2.5 µp;g/well. Blot was blocked with Odyssey Blocking Buffer (TBS) and probed pAkt Rabbit mAb (Santa Cruz P/N sc-13560) and Akt mAb (CST P/N 2967). Signal was detected with IRDye® 800CW Goat anti-Rabbit IgG (LI-COR P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (LI-COR P/N 926-68070) and scanned on Odyssey® CLx Imager (auto scan 700 & 800).